Cryogenic method for extracting cannabinoids Full Spectrum

Full spectrum integrated extractor technique

Plant extraction, practiced for centuries, removes impurities and separates certain elements from plants. Modern science has already identified the terpenes, the flavonoids and more than 400 types of therapeutically active compounds known as cannabinoids.

Due to the significant synergy between the various cannabinoids and terpenes (scented oils responsible for the aroma of the cannabis plant), it is much more advantageous to use natural preparations made from cannabis, rather than a single purified or synthetic cannabinoid.

According to a study published by CAT Scientific in Germany, using an integrated extractor: "Recent in vitro and animal research using THCA or CBDA extract reveal measurable actions on certain enzymes and certain receptor sites, suggesting potential therapeutic effects for these cannabinoids and requiring the elucidation and refinement of specific extraction techniques which preserve these particular acid forms of these cannabinoids in order to provide material for new experiences and research ”.

As more and more states legalize the therapeutic use of cannabis, a safe and effective methodology is necessary for proper extraction of phytocannabinoids.


Alcohol extraction is the oldest and most common method in botany. With excellent solubility in primary alcohols such as ethanol and isopropanol, cannabinoid extraction is effective as long as there is little water in the alcohol. Before prohibition, ethanol and cannabis tinctures were part of the pharmacopoeia traditional and were readily available for various diseases at the local pharmacist.

Many primary alcohols are suitable for the efficient extraction of cannabinoids but the extraction industry prefers to use ethanol because of its availability, safely handling and its safety and because it does not require high pressure for its extraction. More interestingly, ethanol extraction will produce an extract to full spectrum which will help the synergistic activity of phytocannabinoids to exert an excellent pharmacological activity.

Ethanol contains both polar and non-polar parts and can dissolve hydrophilic and hydrophobic molecules. The polar group of ethanol dissolves hydrophilic compounds such as chlorophyll, pigments and tannins. While the non-polar group of ethanol works with hydrophobic components like plant waxes / lipids, oils, cannabinoids, terpenes and any other substance found in or outside the plant.

On average, cannabis buds produce around 12,5% ​​of the extracted extract. During the extraction, the buds must be completely covered, the ratio of the volume of solvent to the mass must be approximately 20X or more, which reduces the viability of the process and the project because it is necessary to distill a large volume of solvent, ethanol.

Regardless of the solvent used for extraction, careful and appropriate design of the extractor and the extraction process is imperative in the phytocannabinoid extraction industry in order to obtain a better yield with a maximum of cannabinoids without no thermal degradation of the extract. This level of quality must be achieved by playing with temperature parameters and the other physical stages of the extraction process.


Clean Green Biosystems has developed an integrated cryoethanol extraction process after a long 5-year research. The extraction system includes ASME and cGMP complaint handling equipment such as the extractor, shell and tube heat exchanger, condenser, pumps and accessories. The whole process must be operated using PLC-SCADA software. The system is fixed in a shoe coated with stainless steel.

The extractor is a double-shelled tank in stainless steel 316 with a high-torque stirring system with FLP motor and a gearbox, the speed of which must be regulated by a VFD. The base of the extractor is removable using a hydraulic power pack which facilitates the simple disposal of cannabis waste. A recirculation line is available using an FLP pump with VFD, to regulate the flow of the miscella.

A suitable shell and tube heat exchanger is the part of the system through which ethanol enters the extractor. Liquid nitrogen passes through the shell side of the heat exchanger which cools the ethanol to the cryoprecipitation temperature. This liquid nitrogen is recirculated using a liquid nitrogen transducer. Liquid nitrogen can also be routed to the extractor shell to maintain the process temperature.

The frozen and reduced-size cannabis plant material is loaded into the extractor. The size of the cannabis plant material is critical in this case. Simultaneously, the cryoethanol is sent to the extractor. The programmed automaton starts the agitator at a given time. After a few hours of extraction, recirculation begins to ensure very efficient extraction. The integration of agitation and recirculation maximizes the efficiency of the extraction. Once the extraction is complete, the system stops and the recirculation pump collects the cannabinoids rich in micelle in a tank. This micelle is taken for further processing.

Which solvent to use?

MZ12X is the only purified extraction solvent for the extraction of medicinal plants with a purity of 99,996% and 12 times decanted.

Compared to butane, dimethyl ether is safer than butane for the end user, it is less explosive and is less harmful to health.
Le DME is not a petroleum-based product. It comes from natural sources of methane. Mz12X is currently distributed in Europe in 4 countries: France, United Kingdom, Netherlands, Croatia.


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Tags : cannabinoidsExtractionphytocannabinoidsPhytotherapyTechnologyterpene